Glycoprotein Labeling with Click Chemistry (GLCC)

Specific and quantitative labeling of biomolecules using click chemistry

Specific and highly efficient fluorescent labeling techniques for biomolecules, especially for proteins, are required for the quantitative analyses of bio-phenomena and for subsequent systems biology. Although expression of exogenous proteins fused with fluorescent tags, such as green fluorescent protein, is the most widely used method for quantitative bio-analysis, the following problems need ...

"Four-potential" ferrocene labeling of PNA oligomers via click chemistry.

The scope of the Cu(I)-catalyzed [2 + 3] azide/alkyne cycloaddition (CuAAC, click chemistry) as a key reaction for the conjugation of ferrocene derivatives to N-terminal functionalized PNA oligomers is explored herein (PNA: peptide nucleic acid). The facile solid-phase synthesis of N-terminal azide or alkyne-functionalized PNA oligomer precursors and their cycloaddition with azidoferrocene, eth...

Membrane labeling and immobilization via copper-free click chemistry.

Copper-free click chemistry was employed to derivatize membrane bilayers. This approach uses an azido-lipid conjugate presented on liposomes, which can be labeled in bioorthogonal fashion via cyclooctyne-tagged reagents. An immobilization-based approach using streptavidin-coated microplates was exploited to evaluate membrane derivatization.

Click chemistry with DNAw

Fast, cell-compatible click chemistry with copper-chelating azides for biomolecular labeling.

The copper-catalyzed azide–alkyne cycloaddition, or CuAAC, has been used extensively for the conjugation, immobilization, and purification of biomolecules. Despite excellent reaction kinetics, high specificity, and bioorthogonality, CuAAC has been used to a far lesser extent in the cellular context because of toxicity caused by the Cumediated generation of reactive oxygen species (ROS) from O2....